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ap2α staining  (Human Protein Atlas)

 
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    Human Protein Atlas ap2α staining
    A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and <t>AP2γ</t> stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).
    Ap2α Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap2α staining/product/Human Protein Atlas
    Average 90 stars, based on 1 article reviews
    ap2α staining - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma"

    Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06733-3

    A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).
    Figure Legend Snippet: A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).

    Techniques Used: RNA Sequencing, Expressing, Immunohistochemical staining, Staining, Derivative Assay, Two Tailed Test

    A Mel Im cell growth over 7 days (Scale bar = 200 µm). B Cell cycle analysis of Mel Im FUCCI in CIB and MG, respectively. C Mel Im migration in CIB during cultivation (Scale bar: 200 µm). D mRNA-expression analysis for AP2α, AP2γ, and AP2ε in MG and CIB ( n = 3 ). E Immunohistochemical staining for AP2ε in Mel Im cells bioprinted in CIB and MG. F Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct and afterwards cultivated in MG or CIB ( n = 3 ). G Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct after transfection of siRNA against AP2ε and control-siRNA, respectively. After transfection cells were cultivated in MG ( n = 3 ) or CIB ( n = 4 ). H mRNA-expression analysis for AP2ε after cultivating the melanoma cell lines Mel Im and 501 Mel under hypoxic conditions ( n = 3 ). I Immunofluorescence images of AP2ε and HIF1-α or Ki-67 in the human melanoma tissue samples (Magnification: 40x). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05; ns : not significant; (Two-tailed Student’s t-test) ( D , F , G ); (Scale bars ( A , C , E , I ): 10 µm).
    Figure Legend Snippet: A Mel Im cell growth over 7 days (Scale bar = 200 µm). B Cell cycle analysis of Mel Im FUCCI in CIB and MG, respectively. C Mel Im migration in CIB during cultivation (Scale bar: 200 µm). D mRNA-expression analysis for AP2α, AP2γ, and AP2ε in MG and CIB ( n = 3 ). E Immunohistochemical staining for AP2ε in Mel Im cells bioprinted in CIB and MG. F Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct and afterwards cultivated in MG or CIB ( n = 3 ). G Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct after transfection of siRNA against AP2ε and control-siRNA, respectively. After transfection cells were cultivated in MG ( n = 3 ) or CIB ( n = 4 ). H mRNA-expression analysis for AP2ε after cultivating the melanoma cell lines Mel Im and 501 Mel under hypoxic conditions ( n = 3 ). I Immunofluorescence images of AP2ε and HIF1-α or Ki-67 in the human melanoma tissue samples (Magnification: 40x). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05; ns : not significant; (Two-tailed Student’s t-test) ( D , F , G ); (Scale bars ( A , C , E , I ): 10 µm).

    Techniques Used: Cell Cycle Assay, Migration, Expressing, Immunohistochemical staining, Staining, Luciferase, Activity Assay, Transfection, Construct, Control, Immunofluorescence, Two Tailed Test

    A Representative AP2ε immunohistochemical analysis of lung metastasis from Tg(GRM1) and AP2ε -/- /Tg(GRM1) mice. a, b, c, d Red arrows depicting AP2ε positive nuclear stainings. Number of disseminated cells per field of view of 3 mice per genotype in 10 sections per mice have been counted ( n = 3). (Scale bar: 200 µm). B Migratory behavior of AP2ε -/- /Tg(GRM1) cells compared to Tg(GRM 1 ) using the Boyden chamber model. ( n = 3). C Attachment analyses by using the xCELLigence system of Tg(GRM1) and AP2ε -/- /Tg(GRM1) for cells from metastatic lung tissue (Delta Cell index = relative change in measured impedance to represent cell status) ( n = 3). D Migratory behavior of Mel Im cells transfected with the AP2ε-overexpression plasmid compared to control vector pCMX using the Boyden chamber model. ( n = 3). E Relative luciferase activity in primary AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells, respectively, transfected with an AP2-Luciferase construct and afterwards cultivated in CIB ( n = 3 ). F Spheroid outgrowth assay depicting reduced migratory activity in AP2ε -/- /Tg(GRM1) spheroids. Quantification of the distance of outgrowth in µm. G Embedded AP2ε -/- /Tg(GRM1) and Tg(GRM1) spheroids after 14 days in MG. White arrows depicting outgrown cells from the Tg(GRM1) spheroid. Protrusion lengths of 9 spheroids per condition have been counted after 10 days in MG ( n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05 (two-tailed Student’s t-test) ( B , C , D , E ); For A and G an unpaired t-test with Welch’s correction was applied; number of experimental animals ( A ) n = 3.
    Figure Legend Snippet: A Representative AP2ε immunohistochemical analysis of lung metastasis from Tg(GRM1) and AP2ε -/- /Tg(GRM1) mice. a, b, c, d Red arrows depicting AP2ε positive nuclear stainings. Number of disseminated cells per field of view of 3 mice per genotype in 10 sections per mice have been counted ( n = 3). (Scale bar: 200 µm). B Migratory behavior of AP2ε -/- /Tg(GRM1) cells compared to Tg(GRM 1 ) using the Boyden chamber model. ( n = 3). C Attachment analyses by using the xCELLigence system of Tg(GRM1) and AP2ε -/- /Tg(GRM1) for cells from metastatic lung tissue (Delta Cell index = relative change in measured impedance to represent cell status) ( n = 3). D Migratory behavior of Mel Im cells transfected with the AP2ε-overexpression plasmid compared to control vector pCMX using the Boyden chamber model. ( n = 3). E Relative luciferase activity in primary AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells, respectively, transfected with an AP2-Luciferase construct and afterwards cultivated in CIB ( n = 3 ). F Spheroid outgrowth assay depicting reduced migratory activity in AP2ε -/- /Tg(GRM1) spheroids. Quantification of the distance of outgrowth in µm. G Embedded AP2ε -/- /Tg(GRM1) and Tg(GRM1) spheroids after 14 days in MG. White arrows depicting outgrown cells from the Tg(GRM1) spheroid. Protrusion lengths of 9 spheroids per condition have been counted after 10 days in MG ( n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05 (two-tailed Student’s t-test) ( B , C , D , E ); For A and G an unpaired t-test with Welch’s correction was applied; number of experimental animals ( A ) n = 3.

    Techniques Used: Immunohistochemical staining, Transfection, Over Expression, Plasmid Preparation, Control, Luciferase, Activity Assay, Construct, Two Tailed Test



    Similar Products

    ap2α staining  (Human Protein Atlas)
    90
    Human Protein Atlas ap2α staining
    A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and <t>AP2γ</t> stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).
    Ap2α Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ap2α staining/product/Human Protein Atlas
    Average 90 stars, based on 1 article reviews
    ap2α staining - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).

    Journal: Cell Death & Disease

    Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma

    doi: 10.1038/s41419-024-06733-3

    Figure Lengend Snippet: A RNA-Sequencing of different melanoma cell lines (SbCl2, WM3211, WM1158, WM793, WM9) depicting a heterogenic expression pattern of the 5 AP2-isoforms. RNA-Seq read counts were normalized to library size. B Immunohistochemical stainings of primary melanoma tumor (PT) or metastasis (Met). AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am). AP2ε was stained with a specific anti-AP2ε-antiserum, established in our group . a, b Image sections depicting high nuclear staining of AP2α in primary tumor and metastasis. c, d Image sections showing weak nuclear staining of AP2γ in primary tumor and low staining in metastasis. e, f Image sections depicting low nuclear staining in primary tumor and only low staining in approximately 5% of the cells in the metastasis for AP2ε. (Scale bar: 200 μm). C Kaplan-Meier survival curve analysis for AP2α, AP2γ, AP2ε in malignant melanoma was performed using the TCGA-derived datasets deposited on the ProteinAtlas database. Survival analysis was performed computationally applying log-rank testing. D Expression analysis for AP2α, AP2γ, and AP2ε published on OncoDB ( https://oncodb.org/index.html ) comparing normal skin tissue (non-tumor) and malignant melanoma (MM) tissue. Data information: Data are represented as mean ± SEM; * p < 0.05 (Two-tailed Student’s t-test).

    Article Snippet: AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am).

    Techniques: RNA Sequencing, Expressing, Immunohistochemical staining, Staining, Derivative Assay, Two Tailed Test

    A Mel Im cell growth over 7 days (Scale bar = 200 µm). B Cell cycle analysis of Mel Im FUCCI in CIB and MG, respectively. C Mel Im migration in CIB during cultivation (Scale bar: 200 µm). D mRNA-expression analysis for AP2α, AP2γ, and AP2ε in MG and CIB ( n = 3 ). E Immunohistochemical staining for AP2ε in Mel Im cells bioprinted in CIB and MG. F Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct and afterwards cultivated in MG or CIB ( n = 3 ). G Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct after transfection of siRNA against AP2ε and control-siRNA, respectively. After transfection cells were cultivated in MG ( n = 3 ) or CIB ( n = 4 ). H mRNA-expression analysis for AP2ε after cultivating the melanoma cell lines Mel Im and 501 Mel under hypoxic conditions ( n = 3 ). I Immunofluorescence images of AP2ε and HIF1-α or Ki-67 in the human melanoma tissue samples (Magnification: 40x). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05; ns : not significant; (Two-tailed Student’s t-test) ( D , F , G ); (Scale bars ( A , C , E , I ): 10 µm).

    Journal: Cell Death & Disease

    Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma

    doi: 10.1038/s41419-024-06733-3

    Figure Lengend Snippet: A Mel Im cell growth over 7 days (Scale bar = 200 µm). B Cell cycle analysis of Mel Im FUCCI in CIB and MG, respectively. C Mel Im migration in CIB during cultivation (Scale bar: 200 µm). D mRNA-expression analysis for AP2α, AP2γ, and AP2ε in MG and CIB ( n = 3 ). E Immunohistochemical staining for AP2ε in Mel Im cells bioprinted in CIB and MG. F Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct and afterwards cultivated in MG or CIB ( n = 3 ). G Relative luciferase activity in Mel Im cells transfected with an AP2-Luciferase construct after transfection of siRNA against AP2ε and control-siRNA, respectively. After transfection cells were cultivated in MG ( n = 3 ) or CIB ( n = 4 ). H mRNA-expression analysis for AP2ε after cultivating the melanoma cell lines Mel Im and 501 Mel under hypoxic conditions ( n = 3 ). I Immunofluorescence images of AP2ε and HIF1-α or Ki-67 in the human melanoma tissue samples (Magnification: 40x). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05; ns : not significant; (Two-tailed Student’s t-test) ( D , F , G ); (Scale bars ( A , C , E , I ): 10 µm).

    Article Snippet: AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am).

    Techniques: Cell Cycle Assay, Migration, Expressing, Immunohistochemical staining, Staining, Luciferase, Activity Assay, Transfection, Construct, Control, Immunofluorescence, Two Tailed Test

    A Representative AP2ε immunohistochemical analysis of lung metastasis from Tg(GRM1) and AP2ε -/- /Tg(GRM1) mice. a, b, c, d Red arrows depicting AP2ε positive nuclear stainings. Number of disseminated cells per field of view of 3 mice per genotype in 10 sections per mice have been counted ( n = 3). (Scale bar: 200 µm). B Migratory behavior of AP2ε -/- /Tg(GRM1) cells compared to Tg(GRM 1 ) using the Boyden chamber model. ( n = 3). C Attachment analyses by using the xCELLigence system of Tg(GRM1) and AP2ε -/- /Tg(GRM1) for cells from metastatic lung tissue (Delta Cell index = relative change in measured impedance to represent cell status) ( n = 3). D Migratory behavior of Mel Im cells transfected with the AP2ε-overexpression plasmid compared to control vector pCMX using the Boyden chamber model. ( n = 3). E Relative luciferase activity in primary AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells, respectively, transfected with an AP2-Luciferase construct and afterwards cultivated in CIB ( n = 3 ). F Spheroid outgrowth assay depicting reduced migratory activity in AP2ε -/- /Tg(GRM1) spheroids. Quantification of the distance of outgrowth in µm. G Embedded AP2ε -/- /Tg(GRM1) and Tg(GRM1) spheroids after 14 days in MG. White arrows depicting outgrown cells from the Tg(GRM1) spheroid. Protrusion lengths of 9 spheroids per condition have been counted after 10 days in MG ( n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05 (two-tailed Student’s t-test) ( B , C , D , E ); For A and G an unpaired t-test with Welch’s correction was applied; number of experimental animals ( A ) n = 3.

    Journal: Cell Death & Disease

    Article Title: Transcription factor activating enhancer-binding protein 2ε (AP2ε) modulates phenotypic plasticity and progression of malignant melanoma

    doi: 10.1038/s41419-024-06733-3

    Figure Lengend Snippet: A Representative AP2ε immunohistochemical analysis of lung metastasis from Tg(GRM1) and AP2ε -/- /Tg(GRM1) mice. a, b, c, d Red arrows depicting AP2ε positive nuclear stainings. Number of disseminated cells per field of view of 3 mice per genotype in 10 sections per mice have been counted ( n = 3). (Scale bar: 200 µm). B Migratory behavior of AP2ε -/- /Tg(GRM1) cells compared to Tg(GRM 1 ) using the Boyden chamber model. ( n = 3). C Attachment analyses by using the xCELLigence system of Tg(GRM1) and AP2ε -/- /Tg(GRM1) for cells from metastatic lung tissue (Delta Cell index = relative change in measured impedance to represent cell status) ( n = 3). D Migratory behavior of Mel Im cells transfected with the AP2ε-overexpression plasmid compared to control vector pCMX using the Boyden chamber model. ( n = 3). E Relative luciferase activity in primary AP2ε -/- /Tg(GRM1) and Tg(GRM1) cells, respectively, transfected with an AP2-Luciferase construct and afterwards cultivated in CIB ( n = 3 ). F Spheroid outgrowth assay depicting reduced migratory activity in AP2ε -/- /Tg(GRM1) spheroids. Quantification of the distance of outgrowth in µm. G Embedded AP2ε -/- /Tg(GRM1) and Tg(GRM1) spheroids after 14 days in MG. White arrows depicting outgrown cells from the Tg(GRM1) spheroid. Protrusion lengths of 9 spheroids per condition have been counted after 10 days in MG ( n = 3). Data information: All data from at least three independent experiments are represented as mean ± SEM; * p < 0.05 (two-tailed Student’s t-test) ( B , C , D , E ); For A and G an unpaired t-test with Welch’s correction was applied; number of experimental animals ( A ) n = 3.

    Article Snippet: AP2α and AP2γ stainings are published by the human protein atlas (AP2α: https://www.proteinatlas.org/ENSG00000137203-TFAP2A/pathology/melanoma AP2γ: https://www.proteinatlas.org/ENSG00000087510-TFAP2C/pathology/melanoma ; accessed on 08/09/2023, 11:51 am).

    Techniques: Immunohistochemical staining, Transfection, Over Expression, Plasmid Preparation, Control, Luciferase, Activity Assay, Construct, Two Tailed Test